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ladder hansenula wingei chromosomes  (Bio-Rad)


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    Structured Review

    Bio-Rad ladder hansenula wingei chromosomes
    Ladder Hansenula Wingei Chromosomes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ladder+hansenula+wingei+chromosomes/CHEF+DNA+Size+Marker/pm36919976-191-24-32
    Average 95 stars, based on 175 article reviews
    ladder hansenula wingei chromosomes - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Pulsed-Field Gel:

    Article Title: Substantial rDNA copy number reductions alter timing of development and produce variable tissue-specific phenotypes in C. elegans.
    Article Snippet: Run conditions were as follows: For long rDNA (∼200 copies or above) (Fig. 1d): 100 V for 68 h, 14 °C, switch times = 300–900 s. For medium-length rDNA (∼70–200 copies) (Fig. 1b): 165 V for 66 h, 14 °C, switch times = 47–170 s. Short rDNA (<70 copies) (Fig. 1c; Supplementary Figs. 4 and 7): 200 V for 24 h, 14 °C, switch times = 15–25 s. After run completion, gel was stained by soaking in ethidium bromide (0.3 μg/mL in 0.5X TBE) to visualize the ladders and imaged on a Bio-Rad GelDoc XR+. .. The following ladders were used in the pulsed-field gel electrophoresis: Fig. 1b ladder: Yeast Chromosome PFG Marker (S. cerevisiae) (NEB #N0345; discontinued); Fig. 1d ladder: Hansenula wingei chromosomes (maximum size 3.13 Mb, Bio-Rad 170-3667); Fig. 1c ladders: 48.5–1,000 kb lambda ladder (Bio-Rad 1703635) (Fig. 1c, right) and 5 kb ladder (Bio-Rad 170-3624) (Fig. 1c, left), which was mixed with 6X DNA loading buffer and loaded as a liquid into well of the short rDNA CHEF gel (all other ladder were loaded as agarose-embedded plugs). ..

    Marker:

    Article Title: Substantial rDNA copy number reductions alter timing of development and produce variable tissue-specific phenotypes in C. elegans.
    Article Snippet: Run conditions were as follows: For long rDNA (∼200 copies or above) (Fig. 1d): 100 V for 68 h, 14 °C, switch times = 300–900 s. For medium-length rDNA (∼70–200 copies) (Fig. 1b): 165 V for 66 h, 14 °C, switch times = 47–170 s. Short rDNA (<70 copies) (Fig. 1c; Supplementary Figs. 4 and 7): 200 V for 24 h, 14 °C, switch times = 15–25 s. After run completion, gel was stained by soaking in ethidium bromide (0.3 μg/mL in 0.5X TBE) to visualize the ladders and imaged on a Bio-Rad GelDoc XR+. .. The following ladders were used in the pulsed-field gel electrophoresis: Fig. 1b ladder: Yeast Chromosome PFG Marker (S. cerevisiae) (NEB #N0345; discontinued); Fig. 1d ladder: Hansenula wingei chromosomes (maximum size 3.13 Mb, Bio-Rad 170-3667); Fig. 1c ladders: 48.5–1,000 kb lambda ladder (Bio-Rad 1703635) (Fig. 1c, right) and 5 kb ladder (Bio-Rad 170-3624) (Fig. 1c, left), which was mixed with 6X DNA loading buffer and loaded as a liquid into well of the short rDNA CHEF gel (all other ladder were loaded as agarose-embedded plugs). ..



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    95
    Bio-Rad ladder hansenula wingei chromosomes
    Ladder Hansenula Wingei Chromosomes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ladder+hansenula+wingei+chromosomes/CHEF+DNA+Size+Marker/pm36919976-191-24-32
    Average 95 stars, based on 1 article reviews
    ladder hansenula wingei chromosomes - by Bioz Stars, 2026-09
    95/100 stars
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